Methylation Analysis Pricing | VCR | University of Nebraska Medical Center

METHYLATION SPECIFIC PCR

Number of samples	1	2	4	8	16
* 1 Primer Set	330	390	485	615	815
Cost per sample	330	195	121	77	51
2 Primer Sets	420	505	630	820	1135
Cost per sample	420	252	157	102	71
3 Primer Sets	510	610	770	1025	1460
Cost per sample	510	305	192	128	91
4 Primer Sets	600	720	910	1230	1780
Cost per sample	600	360	227	153	111
5 Primer Sets	690	830	1050	1430	2100
Cost per sample	690	415	262	178	131
6 Primer Sets	780	940	1200	1630	2420
Cost per sample	780	470	300	203	151

* Based on the number of methylated and unmethylated primer sets per CpG genomic location.

BISULFITE PYROSEQUENCING

Number of samples	1	2	4	8	16
Based on one ~100 bp sequencing per sample	350	420	520	650	845
Cost per sample	350	210	130	81	53

BISULFITE SEQUENCING

Number of samples	1	2	4	8	16
Based on one ~600 bp sequencing per sample.	880	1180	1680	2560	4160
Cost per sample	880	590	420	320	260

METHYL-SENSITIVE CUT COUNTING GENOMIC SEQUENCING (MSCC)

Number of samples	1	2	3	4	5	6
Cost for preparation of number of samples for high throughput sequencing.	720	880	1020	1180	1370	1610
Cost per sample	720	440	340	295	274	268

Note: Additional high throughput sequencing charge of $1050 (Illumina Genome Analyzer) will be added to each sample if sequencing is performed by this facility.

METHYL CpG BINDING DOMAIN - ISOLATED GENOME SEQUENCING (MiGS)

Number of samples	1	2	3	4	5	6
Cost for preparation of number of samples for high throughput sequencing.	835	1190	1565	1930	2295	2660
Cost per sample	835	595	521	482	459	443

Note: Additional high throughput sequencing charge of $1050 (Illumina Genome Analyzer) will be added to each sample if sequencing is performed by this facility.