Advanced Microscopy Core Facility

As a shared research resource supported by the Fred & Pamela Buffett Cancer Center and the NE-INBRE program, the AMCF is a nucleation point for technologies/techniques, expertise, and training, collaboratively leading innovation across research foci.  The Advanced Microscopy Core Facility (AMCF) at the University of Nebraska Medical Center (UNMC) offers a full spectrum of biomedical imaging modalities capable of capturing complex biological phenomena occurring at single molecule (nanometer, super resolution), sub-cellular and cellular resolution (micron, confocal and whole slide scanning), and tissue/small organ levels (millimeter, light sheet). Instruction in the use of, and trained user access to imaging (super resolution, microscopic, mesoscopic) and analysis (HALO, IMARIS) instrumentation are available to internal and external researchers on a fee-for-use basis. With prior approval from the Director, the facility will also collect and/or process images for investigators on a fee-for-service basis.

The AMCF is located on the first floor of the Durham Research Center (DRC I Rooms 1056, 1063, 1036, 1053). Requests for training and instrumentation access may be submitted by emailing or calling (402-559-6659) the facility.

Instrumentation and Services Overview

Instrument details are available on the Equipment Page. Please note this is not an all-inclusive list of services. Contact Director and/or Core staff to discuss individual imaging needs.

• Zeiss Elyra PS.1 Superresolution Microscope
• Zeiss LSM 800 w/ Airyscan for High Resolution Imaging
• Zeiss 710 Confocal Laser Scanning Microscope
• Zeiss Cell Discoverer 7 high Content Plate Reader
• Zeiss Axioscan 7 Whole Slide Imaging System
• UltraMicroscope II Light Sheet Fluorescence Microscope
• X Clarity Automated Tissue Clearing System
• HALO Image Analysis Workstation
• IMARIS Image Analysis Workstation x 2
• Multi-Channel Imaging in fixed or live cells (incubated stages)
• Dynamic Imaging: Time Series, FRAP, FRET, Spectral Imaging, 3D/Volumetric, High-Content Imaging
• Single Molecule Localization Microscopy (PALM, STORM), Structured Illumination Microscopy (SIM)
• Education: In person and on-line researcher resources
• Training: hands-on across imaging modalities
• Consultation: design, collection, analyses

Acknowledging Core Usage

Services and equipment in the AMCF are provided with support from multiple funding agencies and MUST be appropriately cited for sustained operation of these shared resources. AMCF users are obligated to fully acknowledge the facility and its funding sources in formal publications and presentations containing any data generated with support from the facility (instrumentation and/or staff).

Researchers may now use a simplified core acknowledgement statement referencing our Research Resource ID (RRID),

“We acknowledge use of the University of Nebraska Medical Center - UNMC Advanced Microscopy Core Facility, RRID:SCR_022467, P20 GM103427 (NIGMS, NE-INEBRE), P30 GM106397 (NIGMS, NCS), P20GM130447 (NIGMS, CoNDA), P30 CA036727 (NCI, Buffett Cancer Center), S10RR02730 (NIH), S10OD030486 (NIH), Nebraska Research Initiative, UNMC Vice Chancellor for Research Office.”