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UNMC to conduct national clinical study to determine best time during treatment to introduce breast cancer vaccine

The University of Nebraska Medical Center has received a two-year, $500,000

grant to conduct a clinical breast cancer study to test the safety and

effectiveness of a vaccine in combination with surgery and chemotherapy.

The funding was awarded by Avon and the U.S. National Cancer Institute

Progress for Patients Program.

UNMC researchers expect to begin enrolling patients sometime in January.

An estimated 267,000 new cases of breast cancer are expected to occur

among women in the United States during 2003, resulting in an estimated

39,800 deaths.

The vaccine, known as INGN 225, a genetically engineered tumor vaccine,

will be evaluated to treat patients with breast cancer. Two U.S. National

Cancer Institute-designated cancer centers are participating in the gene

therapy trial.

James Talmadge, Ph.D., UNMC professor of pathology and microbiology,

is principal investigator of the study. Patients will be entered into the

study at UNMC by Ken Cowan, M.D., Ph.D., director of UNMC Eppley Cancer

Center, and Elizabeth Reed, M.D., associate professor of internal medicine

and director of the UNMC Breast Cancer Program.

H. Lee Moffitt Cancer Center in Tampa, Fla., also will participate in

the study by preparing the vaccine and participating in immunologic evaluations.

The vaccine is manufactured by Introgen Therapeutics, Inc., of Austin,

Texas.

Dr. Cowan said the vaccine will provide a surveillance system, which

researchers hope will eradicate any tumor cells left following treatment.

The vaccine in essence would mop up any tumor cells that are left behind

using the bodys immune system, Dr. Cowan said.

Dr. Talmadge said the uniqueness of the study is that it will establish

when during the course of a patients treatment it is best to introduce

the vaccine so it will be most effective. He knows of no other studies

looking at the timing of introducing cancer vaccines during treatment.

He said most vaccine protocols are begun following several cycles of chemotherapy.

This is a unique and very important question, said Dr. Talmadge, whos

been working with breast cancer vaccines for the last five years. Im

pleased with the protocol that we developed and its potential to help advance

vaccine therapy for cancer. If the question of vaccine timing relative

to primary chemotherapy is successfully addressed, the results could be

relevant to other tumor types undergoing vaccine therapy.

In other studies, we accept patients independent of the extent of prior

therapy and so some respond, and some dont. We dont understand why the

vaccine doesnt work with all patients and the variability in the extent

of prior therapy likely contributes to the different response rates, Dr.

Talmadge said.

Researchers theorize that vaccinating patients earlier may result in

a higher immune response to the vaccine. The vaccine is given with the

hope of preventing recurrence of breast cancer.

A better understanding of when to give the vaccine relative to primary

therapy should improve the patients immune response to the therapy and

enable us to move on to more effective protocols and vaccines, Dr. Talmadge

said.

In the randomized study, which will begin enrollment in January, one

group of patients will receive a total of four vaccine injections during

and following chemotherapy, and the other will receive a total of four

vaccinations following completion of chemotherapy and radiation. Fifty

patients, with stage II or stage III breast cancer, who have not yet begun

surgery, chemotherapy and/or radiation treatment, may be enrolled for the

study. Stage II and III breast cancer patients are considered those with

four or more lymph nodes with metastasis, and/or a tumor the size of three

centimeters.

The vaccine was developed based on work by Dr. Dmitry Gabrilovich, associate

professor of oncology at the Moffitt Cancer Center, and Dr. David Carbone,

professor of oncology at the Vanderbilt-Ingram Cancer Center, and is exclusively

licensed to Introgen Therapeutics. Previously published pre-clinical data

from Dr. Gabrilovichs and Dr. Talmadges laboratories have shown that

animals vaccinated with p53 vectors are protected against tumor development.

INGN 225 is already being evaluated in patients with small-cell lung cancer

at Moffitt Cancer Center, in a phase I study.

Advexin therapy, recently designated as a Fast Track Program for head

and neck cancer by the FDA, has been evaluated in numerous clinical studies

including phase 3 studies for head and neck cancer, as well as phase 1

and 2 studies for various types of cancer.  In these studies, Advexin

uses the p53 protector gene (guardian of the genome) to directly kill

cancer cells and to stop tumor growth, without harming normal cells.