New UNMC microscope first of its kind in the state

David Kelly Ph.D.

A critical new fluorescence microscope and reader now provides researchers at UNMC the ability to capture images of cells and to analyze changes in their size, shape, motility, intensity and other cellular properties.

Paired with a fully-automated robotics platform, high-throughput (HTP) cell imaging screens identify potential genetic therapeutic targets or candidate drugs for therapy and can perform almost around-the-clock.

According to David Kelly, Ph.D., research assistant professor and director of the Eppley Institute High-Throughput Screening Facility, this technology was previously unavailable in Nebraska and can now be used to study any cell type, including bacteria, yeast, animal and human.

“Measurement of 60,000 cells across hundreds of different experimental treatments can be made in less than 15 minutes,” Dr. Kelly said. “The critical and rate-limiting step in the use of HTP screening is the availability of analytical techniques that allow assessment of the properties imparted to cells by genetic manipulation, like gene silencing or drug treatment.

“This capability greatly enhances the competitiveness of research in medicine, drug development and infectious diseases.”

The fluorescence microscope was made possible by a $250,000 award from the University of Nebraska Foundation annual grants program and $85,000 from Eppley Cancer Center.

The University of Nebraska Foundation’s board of directors awarded eight grants, including the UNMC grant, across the university totaling $1.14 million for 2008-2009. Much of the annual grant resources are made available from unrestricted donations to the foundation.

“We are pleased with this and other programs funded this year, as they help make the university an attractive choice for education and employment,” said Brian Hamilton of Grand Island, chairman of the foundation’s grants committee. “The high level of research and quality education at UNMC is invaluable to our future.”

The University of Nebraska Foundation is an independent, nonprofit organization raising private gifts to support the University of Nebraska for more than 72 years. More than $130 million — the most ever — was provided last year for students, faculty, academic programs, research and building and campus capital improvements on all four campuses.

More information is available at www.nufoundation.org.