Core Facility Focus — High-Throughput DNA Sequencing and Genotyping Core Facility

The High-Throughput DNA Sequencing and Genotyping Core Facility features this Applied Biosystems 3730 Capillary Electrophoresis DNA Analysis System — a state-of-the art DNA sequencer with the ability to sequence up to 700 to 800 base pairs of DNA with greater than 98 percent accuracy depending upon the template.

Today we take a look at the High-Throughput DNA Sequencing and Genotyping Core Facility, which is one of several core facilities available for use by UNMC researchers.

Core facilities, sometimes called shared resources, provide sophisticated, expensive technology and expertise that no single lab would have the financial resources to purchase and learn. These fee-for-service resources help investigators maximize productivity by providing access to technologies, services and scientific consultation at a reasonable cost. Core facilities also provide stability, reliability, cost-effectiveness and quality control that would be difficult to achieve otherwise.

Core facility name: High-Throughput DNA Sequencing and Genotyping Core Facility

Core facility location: 20120 Munroe-Meyer Institute

Services offered: DNA Sequencing and Microsatellite Genotyping. The facility provides 24-hour turn around service for sequencing of a variety of DNA substrates including PCR products, cDNAs and genomic fragments. Microsatellite genotyping also can be performed for genetic linkage studies.

Featured equipment: Applied Biosystems 3730 Capillary Electrophoresis DNA Analysis System. This is a state-of-the art DNA sequencer with the ability to sequence up to 700 to 800 base pairs of DNA with greater than 98 percent accuracy depending upon the template.

Testimonials: The core facility serves approximately 73 different laboratories at UNMC annually and processes more than 20,000 DNA samples a year. This service is crucial to the research enterprise at UNMC. We are able to provide this service with rapid turn around time and a high degree of proficiency as we have great equipment, excellent staff in the facility and great customers.

Core facility contact: Jim Eudy, Ph.D., at 559-3940

Core facility managers wishing to have a focus piece done on their facilities can send an e-mail to today@unmc.edu with the facility’s name, location, services offered, featured equipment, testimonials, manager contact information and a photo of the facility.