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Structural Biology Shared Resource

Structural Biology

Supported by the Fred & Pamela Buffett Cancer Center (FPBCC), the Nuclear Magnetic Resonance Spectroscopy and Crystallography services are key components of the Structural Biology Shared Resources. These resources play a crucial role in supporting the Basic Science research community at UNMC by supporting molecular-level research studies. 

Structural Biology Shared Resource Specific Aims: 

  • Apply structural techniques to analyze essential cancer-related biological macromolecules 
  • Supports in gathering basic knowledge of disease mechanisms 
  • Drives research and directs the synthesis of novel therapeutics 

 Equipment: 

  • 3x AKTA pure chromatography systems. 
  • BioFlo 4500 fermenter/bioreactor 
  • ProteinSimple Peggy Sue 
  • Freedom EVO 150 
  • Formulatrix RockImager 
  • Art Robbins Gryphon Crystal Tray Robot 
  • Nicoya OpenSPR Rev 4 
  • Nanotemper Prometheus Panta 
  • Rigaku BioSAXS 1000 
  • Rigaku FRE+ SuperBright X-Ray source 
  • Rigaku R-Axis HTC detector 
  • 80 Core , 400 GB Ram, 65 TB Array, Data processing Server 
  • 400 MHz Bruker NMR System 
  • 600 MHz Bruker NMR System 

 Services: 

  • Protein Expression and Purification (PrEP) – The PrEP service is dedicated to the high-yield production, purification, and labeling of protein which is used by investigators for a wide range of purposes. 
  • Crystal Screening and Growth (CSG) – The CSG service uses the Formulatrix Rockmaker LIMS and associated robotic instrumentation to guide the crystallization of proteins of interest. 
  • X-ray – The X-ray service has a single crystal diffractometer for solving high-resolution X-ray crystal structures.  The system consists of an FRE+ rotating copper anode generator, high resolution optics, quarter-chi goniometer, and an R-Axis HTC detector.  The service also has a Rigaku BioSAXS-1000 to collect Small Angle X-ray Scattering (SAXS) data on solutions. 
  • Nuclear Magnetic Resonance (NMR) – The NMR service operates spectrometers (400 and 600 Mhz) for verification of organic synthesis of small molecule inhibitors. 

Find more information on: Services, Pricing, and Request Form.  

Impact

Structural Biology

The Structural Biology Shared Resource is located in the FPBBC and the ESH (600 MHz NMR) allowing 80% of the FPBCC members to have easy access.

The Core has facilitated some of the remarkable research studies, including the development of SMI Therapeutic for Multiple Myeloma, aimed at creating new drugs with improved cellular activity, the investigation of Structural Inhibition of MnSOD by H2O2, exploring how mutations can lead to diseases like cancer and other studies. 

Director

Gloria E.O. Borgstahl, PhD

Director, Structural Biology Shared Resource