Molecular Biology Shared Resource
Supported by the Fred & Pamela Buffett Cancer Center (FPBCC), the Molecular Biology Shared Resource provides genomics services including Next Generation sequencing and Sanger DNA sequencing services; single cell genomics assays by 10x Genomics, and Nanostring technology targeted gene expression assays.
Molecular Biology Shared Resource Specific Aims:
- Provide functional genomics services to the FPBCC and other investigators throughout Nebraska.
- Train and mentor users on genomic services.
- Continue to develop/update new procedures and instrumentation to assist FPBCC and other researchers.
Equipment:
- NovaSeq 6000, NextSeq550, MiSeq
- SciClone Liquid Handler
- 10x Genomics X
- Fragment Analyzer - AATI
- Nanostring Encounter
Services:
- NGS sequencing
- NGS library construction
- Single Cell Genomics – scRNA
- RNA and DNA quality control
- Gene Expression Panel
- Sanger Sequencing – (Discontinued as of July 1, 2023).
Find more information on: Services, Pricing, and Request Form.
Impact
The Molecular Biology Shared Resource is located in Durham Research Center II, which allows 80% of the FPBCC members easy access.
The BSR manages all the NGS data. The BSR transfers files to users and, in many instances, performs data analysis for the users. The core also supports research studies when performing single-cell genomics experiments.
91 manuscripts acknowledged the Core, with 36 of them authored by members of the Cancer Center.
Director
Director, Molecular Biology Shared Resource