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University of Nebraska Medical Center

Flow Cytometry Shared Resource

Flow Cytometry

Supported by the Fred & Pamela Buffett Cancer Center (FPBCC) the Flow Cytometry Shared Resource provides UNMC researchers and surrounding institutions with a central location for flow cytometry instrumentation and education. 

Flow Cytometry Shared Resource Specific Aims: 

  • Provide access to flow cytometry instrumentation and services, through a fee-for-service shared resources model, to investigators in the Buffett Cancer Center (BCC) and the UNMC community, as well as other regional academic and corporate clients. 
  • Provide education, training, and support to investigators in the experimental design, sample preparation, acquisition, analysis, and presentation of flow cytometry data. 
  • Provide expertise and comprehensive flow cytometry support to enhance collaborative development of assays and new applications guided by investigator needs. 

Equipment: 

  • ThermoFisher Bigfoot Spectral Cell Sorter  
  • BD FACS AriaII Cell Sorter (x2) 
  • BD Fortessa X-50 Analyzer 
  • BD LSRII (x2) 
  • ThermoFisher Attune NxTAnalyzer 
  • Cytek Northern Lights Spectral Analyzer 

Services: 

  • High-Speed Cell Sorting 
  • Data Acquisition 
  • Data Analysis 

 Additional Services 

  • Training/Education 
  • Figure Generation for Publication 
  • Consultations 
  • Other 

Find more information on: Services, Pricing and Request form.  

Impact

Flow Cytometry

The Flow Cytometry Shared Resource, located in Durham Research Center I, provides convenient access for 80% of FPBCC members.

The Core has supported 128 publications and contributed to numerous research studies by offering high-speed cell sorting, data acquisition and analysis, as well as training and guidance in flow cytometry during data collection. 

Director

Craig L. Semerad, PhD

Director, Flow Cytometry Shared Resource