Dr. Chand's Laboratory
Subhash Chand, PhD
Research Statement
HIV infection increases the chances of heart failure by twofold. Even in people living with HIV on combinational antiretroviral therapy, HIV replicates and releases HIV proteins in the circulation, causing systemic inflammation. Thus, people living with HIV have chronic inflammation, and their immune system is continuously activated.
Extracellular vesicles are critical in intercellular communication, inflammation, and disease progression. EVs carry inflammatory cytokines, proteins, and miRNAs in their cargo. Inflammation and HF are strongly interconnected and mutually reinforce each other. Thus, we sought to establish a connection between chronic inflammation to HF via EVs in HIV infection.
Stem cell-based therapeutics have shown that they are effective in treating various diseases. Deeper analyses of stem cell-based therapeutics revealed that the beneficial treatment impacts are mainly due to their paracrine influence via EVs. Also, mesenchymal stem cell-derived EVs have shown that they can repair injured myocardium and improve cardiac function. We plan to tag the MSC-EVs with a cardiac hoping peptide for their efficient delivery to the myocardium. This innovative idea will elucidate the therapeutic potential of MSC-EVs and the targeting ability of CHP to improve HF symptoms in PLWH.
Lab Personnel
Assistant Professor, UNMC Department of Anesthesiology
Research Faculty
Research Technologist I
- HIV infection, substance abuse, and related cardiovascular comorbidities.
- Impact of inflammation and extracellular vesicles in HIV infection-associated comorbidities.
- Modulation in endocannabinoid system in HIV infection and substance use disorders.
Bridge Funding Award ($76,500) - 01/01/2023 - 12/31/2023
Lieberman Research Award ($50,000) - 05/03/2022 - 12/31/2023
Nebraska Center for Nanomedicine Core ($5,000) - 08/01/2022 - 09/30/2023
Mini Sabbatical (MSab) Funding ($2,500) 03/01/2022 - 09/30/2022
Select Publications
- Chand S, DeMarino C, Gowen A, Cowen M, Al-Sharif S, Kashanchi F, Yelamanchili SV, "Methamphetamine Induces the Release of Proadhesive Extracellular Vesicles and Promotes Syncytia Formation: A Potential Role in HIV-1 Neuropathogenesis" (Viruses, 2022) Impact Factor = 4.7. PMID: 35336957; PMCID: PMC8950763; DOI: 3390/v14030550
- Chand S, Gowen A, Savine M, Moore D, Clark A, Huynh W, Wu N, Odegaard K, Weyrich L, Bevins RA, Fox HS, Pendyala G, Yelamanchili SV, "A comprehensive study to delineate the role of an extracellular vesicle-associated microRNA-29a in chronic methamphetamine use disorder Journal of Extracellular Vesicles" (Journal of Extracellular Vesicles, 2021) Impact Factor = 16.0. PMID: 34913274; PMCID: PMC8674191; DOI: 1002/jev2.12177
- Chand S, Jo A, Vellichirammal NN, Gowen A, Guda C, Schaal V, Odegaard K, Lee H, Pendyala G, Yelamanchili SV, "Comprehensive characterization of organ-specific extracellular vesicles protein markers: Implications for preclinical and clinical applications" (ACS Applied Nano Materials, 2020) Impact Factor = 5.9. PMID: 33385108 PMCID: PMC7771629 DOI: 1021/acsanm.0c01654.
- Ananthaswamy N, Fang Q, AlSalmi W, Jain S, Chen Z, Klose T, Sun Y, Liu Y, Mahalingam M, Chand S, Tovanabutra S, Robb ML, Rossmann MG, Rao VB, "A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer" (Nature Communications, 2019). Impact Factor = 16.6. PMID: 30787293; PMCID: PMC6382815; DOI: 1038/s41467-019-08825-7.
- Chand S, Messina EL, AlSalmi W, Ananthaswamy N, Gao G, Uritskiy G, Padilla-Sanchez V, Mahalingam M, Peachman KK, Robb ML, Rao M, Rao VB, "Glycosylation and oligomeric state of envelope protein might influence HIV-1 virion capture by α4β7 integrin" (Virology, 2017). Impact Factor = 3.7. PMID: 28577856. PMCID: PMC5526109. DOI: 1016/j.virol.2017.05.016